Submitted to: Inoculum
Publication Type: Abstract Only
Publication Acceptance Date: 3/10/2005
Publication Date: 8/30/2005
Citation: Beeson, E., Beltz, S.B., Klich, M.A., Bennett, J.W. 2005. Sclerotial production in Aspergillus flavus varies with temperature and nitrogen source. Inoculum. 56(4):9.
Technical Abstract: Twenty strains of Aspergillus flavus from the culture collection of the Southern Regional Research Laboratory, New Orleans, LA, were grown on defined and complex media at 25 degrees C and 37 degrees C for one week. Colonies were screened for sclerotial production, as well as other colony characters including production of conidia, floccose hyphae, and mycelial pigment. When grown on complex media with yeast extract as the nitrogen source, 9 of the strains produced sclerotia at 25 degrees C and 11 produced sclerotia at 37 degrees C. When grown on a defined medium with nitrate as nitrogen source, 8 of the strains produced sclerotia at 37 degrees C and 5 produced sclerotia at 25 degrees C. When ammonium was used as the nitrogen source, only one strain produced sclerotia at 37 degrees C and there was no sclerotial production at 25 degrees C. Sporulation was sparse or absent for all strains grown on ammonium at 37 degrees C. When microarrays of the A. flavus genome become available, these data will be useful in designing conditions for RNA isolation in order to probe the genes involved in sclerotial formation.