|Lee, Nam Sook|
Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 4/6/2005
Publication Date: 6/18/2005
Citation: Roh, M.S., Cheong, E.J., Choi, I-K, Yoo, E-H, Kim, J-Y, Lee, N.S. 2005. Characterization of the Genus Corylopsis. HortScience. 40(4):1051. Interpretive Summary:
Technical Abstract: We investigated the interspecific relationships and intraspecific variations in genus Corylopsis using randomly amplified polymorphic DNA (RAPD) analysis and single nucleotide polymorphism (SNP) in the internal transcribed spacer1(ITS1), 5.8S ribosomal RNA gene specific in C. glabrescens. A total of 86 samples from various botanical gardens, arboreta and from the native sites in Korea and Japan was used. Differences in identification of species between morphological characteristics and RAPD result were noticed in some accessions, such as C. veitchiana (USNA 37208), which clustered together with C. spicata. Although all C. glabrescens, Franch. & Sav., C. coreana Uyeki, and C. glabrescens f. gotoana (Makino) T. Yamanaka accessions clustered in one major group, they could be further divided into five sub-groups and these grouping are not related to the geographical origins. For example, C. coreana accessions from Gwangneung, Mt. Chiri, and Jogye Temple in Korea were clustered closely with C. glabrescens from Honshu, Japan. Further, C. coreana from Namhae, Korea was clustered with C. glabrescens f. gotoana and C. glabrescens from Japan. Although grouping based on SNP data from ITS1, 5.8S ribosomal RNA gene does not agree with that by RAPD markers, it revealed the limitation in identification and classification of the genus due to high intraspecific variations in SNP. Heteromorphic nature of certain species, particularly C. glabrescens made it difficult to identify interspecific relationships or the origin of some accessions, such as ‘Longwood Chimes’. At SNP positions 464, 465, 466, 467, and 496, most accessions of C. glabrescens has C, G, A, A, and T, respectively, while all of these positions was deleted in most accessions of C. coreana. Therefore, it is concluded that C. coreana and C. glabrescens can be regarded as a same species. Although ITS1, 5.8S ribosomal RNA gene can not be used for identification at a sub-species level of C. glabrescens, it proved to be useful to differentiate C. sinensis Hemsl. from C. sinensis var. calvescens Rehder & E. H. Wilson. It is suggested to use C. glabrescens f. gotoana or C. glabrescens f. coreana rather than using C. coreana. We are carrying out analysis based on morphological characters to combine this with the molecular markers that we developed.