|Howell, Charles - Charlie|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2004
Publication Date: 10/2/2004
Citation: Djonovic, S., Dangott, L.J., Howell, C.R., Kenerley, C.M. 2004. Functional characterization of SM1, a 16-kda protein from Trichoderma virens involved in plant-associated interactions [abstract]. American Phytopathological Society. 94(6):S26.
Technical Abstract: Several Trichoderma species are known to induce plant defense responses in different hosts. We have identified a small protein (SM1) of 16-kDa from culture filtrates of Trichoderma virens. SM1 was purified in a native form to homogeneity by anion-exchange and gel filtration chromatography. Purified protein induced HR-like lesions and autofluorescence response on Oryza sativa and Nicotiana tabacum leaves. SM1 was bioassayed for toxicity against several plant pathogenic fungi and bacteria; however no inhibition of growth was observed. Antibodies for SM1 have been raised and strains disrupted in the sm1 gene were obtained. The targeted gene disruption was conducted via fungal transformation of an arginine auxotroph, and confirmed by Southern, Northern and SDS-PAGE. By conducting various plant bio-assays, we are currently testing the ability of SM1 to induce plant defense responses and to protect plants challenged with soilborne fungi Pythium ultimum and Rhizoctonia solani.