Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2005
Publication Date: 7/11/2005
Citation: Wang, S.Y., Feng, R., Bowman, L., Penhallegon, R., Min, D. 2005 Inhibitory effect of lingonberries (vaccinium vitis-idaea l) extracts on activator protein 1, nuclear factor-kappab, and mitogen-activated protein kinases (mapks). Hortscience. 40(4):1090 Interpretive Summary:
Technical Abstract: Inhibitory Effect of Lingonberry (Vaccinium vitis-idaea L) Extracts on Activator Protein -1, Nuclear Factor-KappaB, and Mitogen-activated Protein Kinases S. Y. Wang,*1 R . Feng2, L. L. Bowman,2 R. Penhallegon3 and M. Ding2 1Fruit Laboratory, Beltsville Agricultural Research Center, ARS,, U. S D.A., Beltsville, Maryland 20705; 2Pathology and Physiology Research Branch, Health Effects Laboratory Division, NIOSH, Morgantown, WV 26505;3 Oregon State University/ Lane County Extension, Eugene, Oregon 97402-3913 The effects of lingonberry (Vaccinium vitis-idaea L) extracts on activator protein -1, nuclear factor-kappaB, and mitogen-activated protein kinases (MAPKs) were evaluated. Pretreatment of JB6 P+ mouse epidermal cells with lingonberry extracts produced a dose-dependent inhibition of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-'B) induced by either 12-O-tetradecanoylphorbol-13-acetate (TPA) or ultraviolet-B (UVB) light. Lingonberry extracts blocked UVB-induced phosphorylation of the mitogen-activated protein kinase (MAPK) family members ERK1, ERK2, and p38 but not JNK. Lingonberry extracts also prevented TPA-induced phosphorylation of ERK1 and ERK2. Results of soft agar assays indicated that lingonberry extracts suppressed TPA-induced neoplastic transformation of JB6 P+ cells in a dose-dependent manner. Lingonberry extracts also induced the apoptosis of human leukemia HL-60 cells in a dose-independent manner. These results suggest that ERK1 and ERK2 may be inhibited by lingonberries, which results in suppression of AP-1 and neoplastic transformation in JB6 P+ cells and causes cancer cell death by an apoptotic mechanism in human leukemia HL-60 cells.