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United States Department of Agriculture

Agricultural Research Service


item Lundgren, Jonathan
item Duan, Jian
item Paradise, Mark
item Wiedenmann, Robert

Submitted to: Journal of Entomological Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/28/2004
Publication Date: 4/1/2005
Citation: Lundgren, J.G., Duan, J.J., Paradise, M.S., Wiedenmann, R.N. 2005. Rearing protocol and life history traits for poecilus chalcites (coleoptera: carabidae) in the laboratory. Journal of Entomological Science, 40(2): 126-135.

Interpretive Summary: Ground beetles are important as biological control of insect and weed pests, but our knowledge of their biology is hampered by the difficulty in rearing them in the laboratory. A rearing protocol was devised for an abundant species of ground beetle, Poecilus chalcites, and various components of the rearing protocol were examined to determine whether improvements were possible. Soil moisture levels, components in the artificial diet, and substrate selection all had important effects on larval development. A method for rearing nearly 80% of larvae to pupation is described.

Technical Abstract: A rearing protocol for the predaceous ground beetle, Poecilus chalcites (Say), is described. The effects of dietary constituents, substrate moisture content, and substrate type on larval developmental rates and size were examined in the laboratory. The protocol was successful in obtaining nearly 80% pupation rates, although adult size was smaller than field-collected beetles, and laboratory-produced adults did not lay eggs. We determined experimentally that some of the components of the meridic diet used for colony production could be removed without compromising larval size or developmental rates, but that nutrition beyond cat food was necessary to increase larval size. We found a positive correlation of larval size with increased moisture content using vermiculite substrate at three moisture levels (33.3, 50.0 and 66.7% by weight). Untreated Fer-Til® (GreenGro Products, Jackson, WI) soil resulted in the highest pupation rate (70 to 80%) and had one of the shortest developmental periods of the five soils tested. Steaming or sifting Fer-Til soil compromised its ability to support larval development until pupation.

Last Modified: 10/19/2017
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