Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 11/23/2006
Publication Date: 9/20/2006
Citation: Tripathi, S., Suzuki, J., Gonsalves, D. 2006. Development of Genetically Engineered Resistant Papaya for Papaya Ringspot Virus in a Timely Manner-A Comprehensive and Successful Approach. In P. Ronald (ed.) Plant-Pathogen Interactions: Methods and Protocols, The Humana Press, Inc., Totowa, New Jersey. P. 197-239. Interpretive Summary: This review provides details on the methodology for developing transgenic papaya for virus resistance, provides an overview of the transgenic papaya work, and discusses the important points that one needs to consider during the development, testing, and practical deployment of transgenic papaya. This is the first review to provide a comprehensive treatment on the methodology and rationale of our approach for developing the transgenic papaya for Hawaii.
Technical Abstract: Papaya orchards throughout most of the world are severely damaged by the destructive disease caused by the papaya ringspot virus (PRSV). PRSV-resistant papaya expressing the coat protein gene (CP) of PRSV have been used in Hawaii to control PRSV since 1998. We present here the experimental steps involved in the development of transgenic papaya including transgene construction, transformation and analysis for virus resistance of the transformed papaya. We also describe the important factors which enabled deregulation, commercialization and adoption of transgenic papaya to occur in Hawaii in a timely manner. Transfer of this technology to other countries with the similar goal and the development of transgenic papaya in other regions of the world is also described here.