Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2004
Publication Date: 11/14/2004
Citation: Konig, G., Gomez, E., Piccone, M.E. 2004. Phylogenetic classification of south americans isolates of the foot and mouth disease virus. Meeting Abstract.
Technical Abstract: Foot and Mouth Disease (FMD) is the economically more prominent disease that affects the domestic animals in South America. The agent etiologic is the Foot and Mouth Disease Virus (FMDV). Phylogenetic analysis allows the characterization of the genetic relations among the different viral strains and provides essential information for the control and the eradication of the disease. The FMDV is classified in seven serotypes and each one of them includes different topotypes. In this work we present the internal classification of the European-South American topotype for the serotypes A, O and C. Through the RT-PCR technique, we amplified the region that codifies for the structural protein 1D (VP1) of 94 South Americans isolates. Subsequently we performed manually nucleotide sequencing of this region by the method of the di-deoxinucleotides. To complete this study, we added 74 sequences of VP1 of South American and European strains previously reported in the Gene bank. We performed the alignment and the phylogenetic analysis of the viral isolates utilizing the programs DNAsis®, Bioedit©, Clustal X, Phylip, NONA© and TreeView©. In the first place we selected the sequences to be used by eliminating those that did not contribute relevant information. Then the European South American topotype of the serotypes A, O and C was internally classified, utilizing as criterion of group the backup of the nodes obtained by the re-sampling technique of the phylograms obtained by the method of distances. The phylogenetic tree of the serotype A, presented seven groups (A-G), belonging the vaccine strains A/Castellanos/ARG/1987, A/ Argentina/2001 and A/Alem/1981 to the groups A, B and C respectively. The phylogenetic tree of the serotype O showed two main groups, the A group contained the isolates O/Argentina/2000 and in the B grouped the vaccine strains O1 Campos/ and O1/Caseros/. Also the serotype C exhibited two fundamental groups, in the A group was located the vaccine strain C3/Resende/ and in the B the vaccine strain C3/Argentina/1985. Employing the method of Maximum Parsimony was determined the origin monophyletic of three groups of the serotype A and one of the serotype C.