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ARS Home » Pacific West Area » Riverside, California » National Clonal Germplasm Repository for Citrus » Research » Publications at this Location » Publication #176173
Title: DEVELOPMENT OF STABLE UNIFORM CONTROLS FOR USE IN ELISA ASSAYS FOR CITRUS TRISTEZA VIRUS

Author
item Lee, Richard
item DEKKERS, M - UNV. OF FLORIDA, CREC
item BAR-JOSEPH, M - VOLCANI CENTER, ISRAEL

Submitted to: International Organization of Citrus Virologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/7/2004
Publication Date: 11/30/2004
Citation: Lee, R.F., Dekkers, M.G., Bar-Joseph, M. 2004. Development of stable uniform controls for use in elisa assays for citrus tristeza virus. 16th Conference International Organization of Citrus Virologists Abstracts, Page 57.

Interpretive Summary:

Technical Abstract: Technical Abstract: Enzyme-linked immunosorbent assays (ELISA) are used almost universally for detection of Citrus tristeza virus (CTV). The positive and negative controls used in ELISA vary greatly from laboratory to laboratory. Even in the same laboratory, there often is a lack of consistency over a period of time. We report a method to prepare standardized positive and negative controls which are relatively stable over a long period of time, even without refrigeration. The use of such standards may permit better direct comparison among laboratories when comparing different ELISA systems for detection of CTV.