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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #175729
Title: VITAMIN E AND GENE EXPRESSION IN IMMUNE CELLS

Author
item Han, Sung Nim
item ADOLFSSON, OSKAR - NESTLE RESEARCH, SWITZERL
item LEE, CHEOL-KOO - UNIV OF WISCONSIN-MADISON
item PROLLA, TOMAS - UNIV OF WISCONSIN-MADISON
item Ordovas, Jose
item Meydani, Simin

Submitted to: Annals of the New York Academy of Sciences
Publication Type: Proceedings
Publication Acceptance Date: 10/1/2004
Publication Date: 12/14/2004
Citation: Han, S., Adolfsson, O., Lee, C., Prolla, T.A., Ordovas, J.M., Meydani, S. 2004. Vitamin E and gene expression in immune cells. In: Annals Of The New York Academy Of Sciences. Vitamin E and Health Symposium, May 22-24, 2004, Boston, Massachusetts, USA. p.96-101.

Interpretive Summary:

Technical Abstract: Aging is associated with dysregulation of immune cells particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging- and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 wks. T cells were purified from splenocytes by negative selection using magnetic beads (anti-Mac-1 and anti-MHC class II), then cultured with media or stimulated with anti-CD3 and anti-CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than 2 fold changes, p<0.05 by ANOVA, and with at least 1 present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.