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United States Department of Agriculture

Agricultural Research Service

Title: THE EFFECT OF GENETICS AND STARVATION ON LYSINE CATABOLISM IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) LIVER)

Author
item Higgins, Ad
item Silverstein, Jeffrey
item Wilson, Me
item Rexroad, Caird
item Blemmings, Kp

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2004
Publication Date: 4/20/2004
Citation: Federation of American Societies for Experimental Biology Conference J. 18(4):A539

Interpretive Summary:

Technical Abstract: This study focuses on lysine catabolism by the lysine alpha-ketoglutarate reductase (LKR) pathway in rainbow trout (RBT). Eight fish from each of four families were randomly allotted to individual tanks. Fish were fed until satiation for 6 wks at which time four fish within each family were randomly selected for 2 wks of starvation. After the 2 wk starvation, all fish were harvested. Hepatic in-vitro LKR activity and lysine oxidation (LOX) were measured as was the LKR mRNA. Strain A exhibited a 55% reduction (P<0.01) in LKR transcripts compared to strain B pooled across both feeding levels. Within each family, LKR mRNA was decreased (P<0.01) in starved versus fed fish. On average, there was a 68% decrease in LKR transcripts for starved fish. LKR activity averaged 104 + 34 and 150 + 31 nmol/min*gm liver (P>0.1) in fed and starved fish, respectively. LOX averaged 0.54 + 0.40 and 0.88 + 0.36 nmol/min*gm liver (P>0.1) in fed and starved fish, respectively. LKR activity was negatively correlated with weight gain (p<0.1) while LKR transcripts were positively correlated to weight gain (p<0.01). These data are consistent with multiple modes of LKR regulation in fish.

Last Modified: 8/24/2016
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