Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2004
Publication Date: 4/20/2004
Citation: Federation of American Societies for Experimental Biology Conference J. 18(4):A539
Technical Abstract: This study focuses on lysine catabolism by the lysine alpha-ketoglutarate reductase (LKR) pathway in rainbow trout (RBT). Eight fish from each of four families were randomly allotted to individual tanks. Fish were fed until satiation for 6 wks at which time four fish within each family were randomly selected for 2 wks of starvation. After the 2 wk starvation, all fish were harvested. Hepatic in-vitro LKR activity and lysine oxidation (LOX) were measured as was the LKR mRNA. Strain A exhibited a 55% reduction (P<0.01) in LKR transcripts compared to strain B pooled across both feeding levels. Within each family, LKR mRNA was decreased (P<0.01) in starved versus fed fish. On average, there was a 68% decrease in LKR transcripts for starved fish. LKR activity averaged 104 + 34 and 150 + 31 nmol/min*gm liver (P>0.1) in fed and starved fish, respectively. LOX averaged 0.54 + 0.40 and 0.88 + 0.36 nmol/min*gm liver (P>0.1) in fed and starved fish, respectively. LKR activity was negatively correlated with weight gain (p<0.1) while LKR transcripts were positively correlated to weight gain (p<0.01). These data are consistent with multiple modes of LKR regulation in fish.