Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/17/2004
Publication Date: 3/1/2005
Citation: Pruett, C., Saillant, E., Renshaw, M., Patton, J., Rexroad Iii, C.E., Gold, J., Microsatellite dna markers for population-genetic studies and parentage assignment cobia, rachyentron canadum. Molecular Ecology Notes 5: 84-86. Interpretive Summary: Cobia (Rachycentron canadum) are important to recreational fisheries in the southeastern United States and have recently become of interest to aquaculture, however, little information is available on the genetics of this species. We developed and characterized twenty DNA markers observed to contain sufficient genetic variation for use in studying population genetics and genetic mapping. Scientists will be able to use these markers to examine wild and captive population structures and in the identification of genes affecting aquaculture production traits.
Technical Abstract: Twenty nuclear-encoded microsatellites from a genomic DNA library of cobia, Rachycentron canadum, were isolated and characterized. The microsatellites include two tetranucleotide, one trinucleotide, three combination tetranucleotide/dinucleotide, nine dinucleotide, and five imperfect (dinucleotide) repeat motifs. Gene diversity ranged between 0 - 0.910; the number of alleles among a sample of 24 fish ranged from 1 - 15. Cobia support an important recreational fishery in the southeastern United States and recently have become of interest to aquaculture. The microsatellites developed will be useful tools for studying both population-genetics (e.g., stock structure, effective population size) and inheritance of traits important to aquaculture.