|Hayes, Michael h|
Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 5/3/2004
Publication Date: 11/1/2004
Citation: Hayes, M.B., Clapp, C.E., Ferreira, J.A., Mao, J.D., Song, G., Byrne, C. 2004. Peptides and saccharides in soil humic substances. ASA-CSSA-SSSA Annual Meeting Abstracts. Paper No. 4779. Interpretive Summary:
Technical Abstract: Diffusion Ordered NMR Spectroscopy (DOSY) has shown that peptides and saccharides are associated with soil humic substances but are not components of (i.e. covalently linked to) the humic molecules. Some, but not all of the peptides/saccharides can be removed from the humic matrix by resin technology. Analyses of the component sugars and amino acids in the hydrolysates of selectively isolated humic fractions show distinct differences in the abundances of the various sugars and amino acids in the fractions. These differences can be related to the origins (plant or microbial) of the parent saccharide and peptide molecules. Applications of XAD-8 [(poly)methylmethacrylate] and XAD-4 (styrenedivinylbenzene) resins in tandem technology, as used for the isolation of humic substances from water, removes from the humic and fulvic acid matrixes some saccharide materials that have microbial rather than plant origins. Amino acids ratios data suggest that the peptide components removed in this way also have microbial origins.