Submitted to: Great Lakes International Imaging and Flow Cytometry Association
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2004
Publication Date: 10/25/2004
Citation: Kimura, K., Harp, J.A., Oesper, J.C., Goff, J.P., Olsen, S.C. 2004. Can lymphocytes from one side of bovine mammary gland migrate to the contralateral gland and lymph node tissue [abstract]. Great Lakes International Imaging and Flow Cytometry Association. p. 17.
Technical Abstract: The 4 quarters of bovine mammary glands are completely separated and 2 quarters on each side (right or left) are connected to ipsilateral supramammary lymph nodes. It is not clear whether cells infused into the lumen of the mammary gland are capable of migrating to lymph nodes or the general circulation. Understanding cell migration is important to prevent/treat mastitis, which is the biggest economic problem to the dairy industry. In order to examine cell migration, a prescapular lymph node was removed from 5 dairy cattle after cessation of milk production and isolated lymphocytes were stained with Hoechst 33342. These stained cells (4-5 x 10**9 cells/25 ml) were infused into the left side of the udder and then activated by intramammary infusion of zymosan-stimulated serum (source of C5a). After 17 h, E. coli J5 bacterin was infused into the contralateral mammary gland to mimic infection. After 26 h (44 h after infusion of cells), cows were euthanized and tissue samples (mammary quarters, right and left supramammary, mesenteric, ileocecal, and prescapular lymph nodes, liver, and spleen) were collected for microscopic examination as well as flow cytometric analysis. Hoechst stained cells were detected not only in infused quarters, but also in contralateral quarters as well as in both supramammary lymph nodes. This indicates that cells infused into the mammary gland migrate to contralateral tissues and supramammary lymph nodes.