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Title: A NOVEL IMMUNOLOGICAL APPROACH FOR QUANTIFYING PREDATION RATES ON GLASSY-WINGED SHARPSHOOTER

Author
item Hagler, James
item Henneberry, Thomas
item DAANE, KENT - U CA, BERKELEY
item FOURNIER, VALERIE - U CA, BERKELEY
item Groves, Russell
item PRABHAKER, NILIMA - U CA, RIVERSIDE
item COSTA, HEATHER - U CA, RIVERSIDE
item HODDLE, MARK - U CA, RIVERSIDE

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: 12/12/2004
Publication Date: 12/12/2004
Citation: Hagler, J.R., Henneberry, T.J., Daane, K., Fournier, V., Groves, R.L., Prabhaker, N., Costa, H., Hoddle, M. 2004. A novel immunological approach for quantifying predation rates on glassy-winged sharpshooter. CDFA Pierce's Disease Control Program Research Symposium. pp. 106-110

Interpretive Summary: A glassy-winged sharpshooter (GWSS) protein marking system is being developed for use as a diagnostic tool for predator gut content analysis. We determined that GWSS can be marked with 100% efficiency for at least 7 days after feeding on protein-marked plant material or spraying with a topical protein solution. Moreover, feeding trials have shown that protein marked insects can be detected by a protein-specific ELISA in the guts of predators that consumed them. Field studies are being initiated that will quantifY the predation rates of an assemblage of predators on GWSS using a multitude of proteinspecific ELISAs.

Technical Abstract: A glassy-winged sharpshooter (GWSS) protein marking system is being developed for use as a diagnostic tool for predator gut content analysis. We determined that GWSS can be marked with 100% efficiency for at least 7 days after feeding on protein-marked plant material or spraying with a topical protein solution. Moreover, feeding trials have shown that protein marked insects can be detected by a protein-specific ELISA in the guts of predators that consumed them. Field studies are being initiated that will quantifY the predation rates of an assemblage of predators on GWSS using a multitude of protein-specific ELISAs.