Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/31/2005
Publication Date: 8/1/2005
Citation: Quiniou, S.M., Wolters, W.R., Waldbieser, G.C. Assignment of xbai repetitive elements to channel catfish centromeres using fish. Animal Genetics. Interpretive Summary: Repetitive DNA sequences, termed 'XbaI elements', have been shown to represent 5-6% of the channel catfish genome, but the location of these sequences was unknown. A DNA probe containing the XbaI element sequence was fluorescently labeled and hybridized to catfish chromosomes. Microscopic visualization of hybridized chromosomes showed these elements were localized to the centromeres of all catfish chromosomes. Knowing the composition and location of centromeres will assist researchers in constructing catfish genome maps, which will help researchers identify genes controlling important production traits.
Technical Abstract: A/T-rich XbaI elements are highly repetitive DNA sequences (321-325bp) that represent 5-6% of the channel catfish genome. The XbaI elements were mapped by fluorescent in situ hybridization to channel catfish metaphase chromosomes. Signals were detected only at the centromere of every chromosome pair with variability in the signal intensity.