Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2005
Publication Date: 4/1/2005
Citation: Lian, F., Russell, R., Wang, X. 2005. Apo-10'-lycopenoic acid, a metabolite of lycopene, inhibits the proliferation of non-small cell lung cancer cells [abstract]. Journal of Federation of American Societies for Experimental Biology. 19:A1458.
Technical Abstract: It has been suggested that the chemopreventive effect of lycopene may be, in part, mediated by its oxidative metabolites. The present study examined the inhibitory effects of apo-10'-lycopenoic acid (ALA), a metabolite of lycopene, on the growth of non-small cell lung cancer cell lines (A549 and NCI-H358). Results showed that the treatment with ALA significantly inhibited the growth of both A549 and NCI-H358 cells in a time- and dose-dependent manner, as determined by cell counting and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry analysis showed that ALA blocked cell cycle progression by increasing cells in G1/G0 phase and decreasing those in S phase in A549 cells exposed to 5 microM of ALA for 48 hours. In addition, pretreatment with 5 microM ALA for 48 hours delayed the activation of extracellular signal-regulated protein kinase (ERK) induced by serum stimulation in starved A549 cells, which is concomitant with the down-regulation of serum-induced cyclin D1 and p21 expression. There was no significant induction of apoptosis as measured by Annexin-V-FLUOS/propedium iodide staining and cleaved PARP. In conclusion, the present study demonstrates that the ALA may mediate the chemopreventive activity of lycopene by inhibiting cell proliferation and modulating ERK signaling pathway.