|Li, Xin Liang|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/14/2005
Publication Date: 1/14/2005
Citation: Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151. Interpretive Summary:
Technical Abstract: We are developing a high throughput plasmid-based functional proteomic workcell that integrates the following operations in a robotic platform: 1) media filling, 2) colony picking, 3) plate sealing, 4) advanced liquid handling, 5) incubation and shaking, and 6) assay data collection into a custom database with 2D bar code tracking of plates, clones, strains, and datapoints. Applications for this technology include the rapid optimization of clones and expression of full-length cDNA libraries to produce improved fuel ethanol yeast strains. Full-length cDNAs libraries are produced using a newly modified liquid handler that is the source of plasmids carrying the optimized sequences for expression in yeast. The final incorporation of this liquid handler in the robotic platform will be capable of running in vitro and in vivo expression reactions to obtain proteins so that clones having optimal traits can be identified before placement into the ethanol fermenting yeast strains. Using the same high throughput platform, the yeast strains can be screened for growth characteristics using standard biochemical assays. This poster will discuss the design, construction, and validation of these systems.