|O Donnell, Kerry|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2004
Publication Date: 12/15/2004
Citation: Kistler, H.C., Birren, B., Calvo, S., Cuomo, C., Gale, L.R., Gueldener, U., Ma, L., Muehlbauer, G., O Donnell, K., Trail, F., Ward, T.J., Xu, J. 2004. Genomics of fusarium graminearum. In: Proceedings of the 2nd International Symposium on Fusarium Head Blight, December 11-15, 2004, Orlando, FL, p.4.
Technical Abstract: We have generated a draft sequence assembly of the F. graminearum genome that is available on the web for download and query. The assembly is remarkably complete owing to near lack of repetitive sequences in the genome. After manual editing, the entire assembly currently is contained on 28 scaffolds ranging in size from 3 kb to over 8.8 Mb with an average contig length of over 71 kb. Currently, over 99.8% of the DNA sequence has been anchored to the genetic map by way of 237 genetic markers, 164 of which are sequence-tagged sites. Automated draft gene calls were conducted both at the Broad Institute and at Munich Information Center on Protein Sequences (MIPS) resulting in >11,000 predicted genes. Improvements to the predicted gene sets are being made by manual annotation by members of Gibberella zeae International Genomics Initiative (GIGI), representing over 25 laboratories world-wide and further Expressed Sequence Tag (EST) sequencing at the Broad Institute. MIPS currently is hosting web-access to the manual annotation as well as both the Broad Institute and MIPS gene models. A custom Affymetrix GeneChip microarray designed from gene models derived from the draft assembly is now available. Details of the automated annotation, efforts toward manual annotation, microarray experiments and coordination of functional analysis of the genome will be discussed. The F. graminearum sequencing project is funded by the National Research Initiative (NRI), through the USDA/NSF Microbial Genome Sequencing Program. The MIPS Fusarium graminearum Genome Database is funded by the Austrian Federal Ministry for Education, Science and Culture and the Fusarium microarray is funded by the USDA NRI Integrated Program: Functional Genomics of Microbes.