Submitted to: International Soy Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/8/2004
Publication Date: 10/8/2004
Citation: Glinski, J.A., Davey, M.H., Berhow, M.A. 2004. Separation of soyba saponins reference standards by kromaton fast centrifugal partition chromatography. International Soy Conference.
Technical Abstract: Centrifugal Partition Chromatography (CPC) is an effective yet underutilized tool for purification of compounds naturally produced by plants and microorganisms. CPC separates compounds through partitioning between two immiscible liquid phases of a bi-phasic solvent system. This technique does not involve traditional solid chromatographic materials, known to affect adversely the purification and yields of labile compounds. We have applied Kromaton FCPC (Kromaton Technologies, France) to facilitate the purification of soya sapogenols (A, B, and E) as well as saponins, of the type A and B. A one step fractionation of a mixture of type B saponins in a specifically designed solvent system led to the purification of some saponins and to obtaining highly enriched fractions (50% ' 80%) of other saponins. Additional purification of the enriched fractions requires usually another purification step. The method provides an easy access to rare soya saponins, normally occurring only at very low concentration. The FCPC purification is very well suited for isolating of multi-gram quantities often needed for pharmacological or clinical study.