Submitted to: Plant Cell Reports
Publication Type: Other
Publication Acceptance Date: 7/20/2004
Publication Date: 3/1/2005
Citation: Kim, K.M., Park, Y.H., Kim, C.K., Hirschi, K., Sohn, J.K. 2005. Development of transgenic rice plants overexpressing the arabidopsis h(+)/ca(2+) antiporter cax1 gene. Plant Cell Reports. 23(10-11):678-682. Interpretive Summary: The ability to modify agriculturally important crops is a vital part of agriculturally biotechnology. In this manuscript we detail the ability to express high levels of a calcium transporter in rice. These transgenic rice plants may contain higher levels of calcium and thus be of use in terms of increasing the calcium consumption in the human population.
Technical Abstract: The gene of the Arabidopsis thaliana H(+)/Ca(2+) transporter, CAX1 (cation exchanger 1) was introduced into Japonica cultivars of rice (Ilpumbyeo) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of the CAX1 gene was also proven by PCR using CAX1-specific oligonucleotide primers in regenerated plants. Stable integration and expression of the CAX1 gene in T(0) plants and T(1) progeny were confirmed by DNA hybridization, Northern blot analysis, and luminescent analysis.