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Title: THE SYSTEMIC AND LOCALIZED INNATE IMMUNE RESPONSE TO INTRAMAMMARY INFECTION WITH STREPTOCOCCUS UBERIS

Author
item Bannerman, Douglas
item Paape, Max
item Goff, Jesse
item Kimura, Kayoko
item Lippolis, John
item HOPE, JAYNE - INST FOR ANIMAL HEALTH

Submitted to: Mastitis Council Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/5/2004
Publication Date: 1/18/2005
Citation: Bannerman, D.D., Paape, M.J., Goff, J.P., Kimura, K., Lippolis, J.D., Hope, J.C. 2005. The systemic and localized innate immune response to intramammary infection with streptococcus uberis [abstract]. National Mastitis Council (NMC) 44th Annual Meeting Proceedings. pp. 211-212.

Interpretive Summary: THESE ARE MEETING PROCEEDINGS. NO INTERPRETIVE SUMMARY REQUIRED.

Technical Abstract: Streptococcus uberis is a Gram-positive bacterium that induces clinical mastitis. Once initial host barrier systems have been breached by this pathogen, the innate immune system provides the next level of defense against these infectious agents. The objective of the current study was to characterize the innate immune response during clinical mastitis elicited by S. uberis. The pro-inflammatory cytokine response and changes in the levels of the innate immune accessory recognition proteins, soluble CD14 (sCD14) and lipopolysaccharide (LPS)-binding protein (LBP), were studied. Decreased milk output, induction of a febrile response, and increased acute phase synthesis of LBP were all characteristic of the systemic response to intramammary infection with this organism. Intramammary infection also resulted in localized changes including, increases in milk somatic cells and increased levels of IL-1beta, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha, sCD14, LBP, and the complement component, C5a. Relative to previous reports of other mastitis pathogens, S. uberis infection was characterized by the sustained elevation of higher milk levels of IL-1beta, IL-10, IL-12, IFN-gamma, and C5a. Together, these data identify the temporal changes in the innate immune response to S. uberis infection.