|Holbrook, Carl - Corley|
Submitted to: Multicrop Aflatoxin and Fumonisin Elimination and Fungal Genomics Workshop-The Peanut Foundation
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2004
Publication Date: 10/1/2004
Citation: Liang, X.Q., Guo, B., Holbrook, Jr., C.C., Lynch, R.E. 2004. A nonspecific lipid transfer protein and an allergen ARA H1-like protein are associated with the resistance to Aspergillus sp. in peanut [abstract]. 2004 Fungal Genomics and Aflatoxin/Fumonisin Elimination Workshop, October 25-28, 2004, Sacramento, CA.
Technical Abstract: Peanut genotypes, resistant or susceptible to Aspergillus sp., were extracted with acid buffer, pH 3.0. Although, the antifungal in vitro bioassays showed that protein extracts from all genotypes inhibited A. flavus spore germination and hypha growth, the extracts from all resistant genotypes had stronger antifungal activities than the susceptible genotypes. The protein extract SDS-PAGE profiles revealed that two proteins with molecular weight of 9 kDa and 14 kDa, respectively, was present constitutively in higher concentrations in the seeds of three resistant genotypes. These two proteins were excised from the gels and sequenced using ESI-MS/MS and Edman degradation after trypsin digestion. The sequence suggested that the 9 kDa protein is homologous to the nonspecific lipid transfer proteins 1 (nsLTPs 1). The 14 kDa protein has homology with peanut allergen Ara h1 (vicilin), but the molecular weight is much smaller than peanut Ara h1 protein. It has been reported that LTPs have antifungal activities. These two small proteins may be novel antifungal proteins and contribute to the resistance against Aspergillus flavus in peanut. Further studies of these proteins will be needed in gene expression and regulation.