|Patterson, Kristine - Kris|
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2004
Publication Date: 3/7/2005
Citation: Patterson, B., Wastney, M.E., Combs Jr., G.F., Brindak, M., Patterson, K.Y., Veillon, C., Taylor, P., Levander, O.A. 2005. Se metabolism in humans is altered by long-term supplementation [abstract]. The Federation of American Societies for Experimental Biology Journal. 19(5):A1015. Interpretive Summary:
Technical Abstract: SELECT, an ongoing randomized clinical trial, is testing whether selenium (Se) can prevent prostate cancer; 16,200 men will take 200 ug of Se as selenomethionine (SeMet) daily for 7-12 yrs. We are investigating possible changes in Se metabolism due to long-term supplementation by comparing two 4-mo kinetic tracer studies, the first (PK1) at baseline, the second (PK2) following 2 yrs of supplementation with the drug given in SELECT. Thirty free-living subjects (15 M, 15 F) received oral doses of 2 stable isotope tracers, 150 ug of 74Se as SeMet and 150 ug of 76Se as sodium selenite (Sel). Metabolism before and following supplementation can be compared in the same subjects, removing between-subject variability. Multiple plasma, red blood cell, urine and fecal samples were collected during the 4-mo. study periods. A compartmental model requiring multiple plasma compartments and recirculation of tracer was developed using data from PK1. Based on preliminary results, this model fits both Sel and SeMet, with differing parameter values. Results from several subjects for PK2 suggest that this same compartmental model will fit the data for PK2, but with within-subject differences in some parameter values, but not others. With increased Se intake from ~100 ug/d to ~300 ug/d, fractional absorption of Sel and SeMet did not change. Se absorption and urine excretion of Se increased. We predict that tissue Se increased.