Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/5/2004
Publication Date: 1/20/2005
Citation: Vandemark, G.J., Hughes, T.J., Larsen, R.C. 2005. Genetic similarities between alfalfa cultivars based on sequence related amplified polymorphism (srap) dna markers. Plant and Animal Genome XIII Abstracts. p 102.
Technical Abstract: Our objective was to examine if Sequence related amplified polymorphisms (SRAPs) could be used to detect genetic diversity among a set of alfalfa populations representing original sources of Medicago germplasm and public domain cultivars. Fifteen different populations were examined, including 9 NPGS Plant Introducion accessions representing original Medicago germplasm sources and 6 public domain cultivars. For each population, SRAP reactions were performed using DNA samples extracted from three independent bulk plant samples consisting of 20 seedlings/bulk. Genetic diversity could be quantified both within bulk samples of a population and also between populations. Six different SRAP primer pairs amplified a total of 167 bands between 100 bp'2 kb in length. One hundred thirty-five of the bands (80.8%) were polymorphic among the 45 different bulked DNA samples. The highest similarity between bulk DNA samples within a population was for Vernal(.908)[Fall Dormancy (FD) =2], while the lowest was for PI 536533 (M. varia). The highest similarity between cultivars was 0.659, between Malone (FD = 7) and CUF101(FD = 9), while the least was between Vernal and CUF101 (0.14). The highest similarity between PI accessions was 0.608, between PI536535 (Peruvian) and PI 536536 (Indian). The most distinct PI was PI560333 (M. falcata). SRAPs can clearly detect variation between different alfalfa populations and also between bulked plant samples of the same population. Genetic relationships based on SRAPs appear to be closely correlated with fall dormancy status of evaluated materials.