Submitted to: Genbank
Publication Type: Germplasm Release
Publication Acceptance Date: 8/25/2004
Publication Date: 9/22/2004
Citation: He, G., Meng, R., Guo, B., Dang, P. M. 2004. Nucleotide sequence release of 178 microsatellites in peanut (Accession No. AY731521 to AY731698). GenBank, National Center for Biotechnology Information. Available: http://www.ncbi.nlm.nih.gov.
Technical Abstract: In the U.S., peanut has become the second-most important legume crop, with the U.S. peanut crop having an average farm-gate value of over $1 billion in the 1990s. U.S. peanut production in 2003 was 4.14 billion pounds, and Georgia produced 1.86 billion pounds. Peanut is also a valuable source of protein and oil. However, research has revealed very limited genetic variation at the DNA level. Gene discovery and marker development are needed in cultivated peanut for genetic study and breeding. The development of molecular markers should enable research to measure and exploit variation in the cultivated peanut species. Microsatellites, or simple sequence repeats (SSRs), are stretches of DNA consisting of tandemly repeated short units of 1-6 base pairs in length. The uniqueness and the value of microsatellites arise from their multiallelic nature, codominant inheritance, relative abundance, extensive genome coverage and simple detection by PCR. We have constructed microsatellite enriched genomic libraries and sequenced about 5,000 clones. Release of these nucleotide sequences of 178 microsatellite (accession No. AY731521 to AY731698) to the public database, GenBank (National Center for Biotechnology Information), will promote the interest in utilization and development of microsatellite markers in cultivated peanuts. These markers are valuable in cultivar identification, genetic mapping, and molecular breeding. Identification of the genes associated with the resistance traits and development of molecular markers associated with the resistance will bring new diversity into peanut gene pools and expedite the development of new peanut cultivars with the desired characteristics.