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Title: XYLITOL PRODUCTION FROM L-ARABINOSE

Author
item SAKAKIBARA, YOSHIKIYO - BRDC
item Saha, Badal
item WYMER, NATHAN - ZUCHEM
item TAYLOR, PAUL - ZUCHEM

Submitted to: UJNR Food & Agricultural Panel Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 11/15/2004
Publication Date: 12/11/2004
Citation: Sakakibara, Y., Saha, B.C., Wymer, N., Taylor, P. 2004. Xylitol production from L-arabinose. In: Cherry, J.P., Pavlath, A.E., editors. Proceedings of the 33rd Annual Meeting of the United States-Japan Cooperative Program in Natural Resources (UJNR), December 11-18, 2004, Honolulu, Hawaii. p. 191-194.

Interpretive Summary:

Technical Abstract: The microbial synthesis of xylitol from L-arabinose, which is one of the major components in hemicellulose, is useful for economical production of xylitol. It is known that fungi metabolize L-arabitol to D-xylulose 5-phosphate via xylitol. We are attempting to clone the genes from L-arabinose utilizing yeasts and to convert L-arabinose to xylitol by using recombinant Escherichia coli. L-xylulose reductase, which is one of three enzymes in the L-arabinose to xylitol pathway, has been cloned from Ambrosiozyma monospora and introduced in E. coli. As a result, this enzyme was functionally expressed in E. coli, and in vivo conversion of xylitol to xylulose (reverse reaction) was observed.