Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/2004
Publication Date: 10/31/2004
Citation: Ulloa, M., Park, Y.H., Alabady, M.S., Stewart, J.M., Wilkins, T. 2004. Orgins of allelic diversity and genic regions revealed by microsatellite est markers in cotton.. ASA-CSSA-SSSA Annual Meeting Abstracts. p. 5869.
Technical Abstract: EST microsatellites were used to elucidate origin of allelic diversity in tetraploid cottons. PCR amplification of microsatellites gave 562 polymorphic and 458 monomorphic DNA fragments among six species. Polymorphic amplicons revealed five scenarios of transferability and rearrangement of amplicons of the allotetraploid AD cotton (G. hirsutum and G. barbadense compared to the progenitor diploid genomes A-(G. herbaceum and G. arboreum) and D-(G. thurberi and G. harknessii). Approximately 8.5% of the microsatellites yielded DNA amplicons from the diploid genomes that matched in size those amplified from both tetraploids; 7.6% gave amplicons from the diploid A or D genomes; and 30.4% amplified amplicons in AD genomes that matched alleles in the diploids but without specific synteny. Partial results indicate that tetraploid cottons underwent a higher rate than expected of DNA rearrangement at the gene level after allopolyploidization. PCR-stable amplicons were 50%, including monomorphic additional diploid and tetraploid species. Genetic relationships among species will be depicted from dendograms constructed from loci-genic sequence information and PCR-DNA amplicons.