Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/2004
Publication Date: 10/31/2004
Citation: Alabady, M.S., Ulloa, M., Park, Y.H., Sickler, B.A., Wilkins, T.A., Stelly, D., Cantrell, R.G. 2004. Cotton (Gossypium arboreum L.) fiber EST-derived compound sequence repeat (CSR) used to develop PCR-based markers. ASA-CSSA-SSA Annual Meeting Abstracts. p. XXX.
Technical Abstract: A new set of microsatellite or CSR markers was developed from the sequence information of a fiber EST database from the diploid species Gossypium arboreum. One thousand EST-CSR sequences were selected were selected based on motif length, gene function notation, and previously mapped EST. Primer design was successful for 624 sequences. A total of 251 (48%) primers showed polymorphisms inter- and/or intra- amoung six species. The polymorphism level between the G. hirsutum and G. barbadense was slightly higher (16.8%) than between G. herbaceum and G. arboreum (7.3%), and G. thurberi and G. harknessii (16.25%). Based on PCR amplification, the transferability of EST-derived CSR markers from the diploid species G. arboreum was high 368 (70.4%) across the six Gossypium species. Chromosomal assignments of 26 markers were determined by using the hypo-aneuploid deficiency G. hirsutum monosomics set. Most of these CSR markers provided information about functional genes. These EST-CSR markers demonstrated portability, strength, and suitability for use in diverse genome analysis. Markers will be used for the development of interspecific linkage maps, evaluation of germplasm collections, and molecular tagging of important traits for marker assisted selection (MAS) in cotton. Primer sequence information of these markers will be available for public use.