Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2005
Publication Date: 7/7/2005
Citation: Liu, X., Gill, B., Chen, M. 2005. Hessian fly resistance gene h13 mapped to a distal cluster of r genes in chromosome 6ds of wheat. Journal of Theoretical and Applied Genetics. V-111: pp. 243-249. Interpretive Summary: Hessian fly is an important pest of wheat that causes death of tillers and lodging. H13 is one of the most effective resistance genes to the Hessian fly. This manuscript reports several markers that can be used in breeding programs for markers-assisted selection. The availability of these markers will accelerate the incorporation of H13 into wheat cultivars for effective control of Hessian fly damage
Technical Abstract: H13 is inherited as a major dominant resistance gene in wheat. It was previously mapped to chromosome 6D and expresses a high level of antibiosis against Hessian fly (Hf) [Mayetiola destructor (Say)] larvae. The objective of this study was to identify tightly linked molecular markers for marker-assisted selection in wheat breeding, and as a good starting point toward the map-based cloning of H13. Fifty-two chromosome 6D-specific microsatellite (simple sequence repeat, SSR) markers were tested for linkage to H13 using near-isogenic lines Molly (PI 562619) and Newton-207, and a segregating population consisting of 192 F2:3 families derived from the cross PI 372129 (Dn4) × Molly (H13). Marker Xcfd132 co-segregated with H13, and several other markers were tightly linked to H13 in the distal region of wheat chromosome 6DS. Deletion analysis assigned H13 to a small region closely proximal to the breakpoint of del6DS-6 (FL0.99). Further evaluation and comparison of the H13-linked markers revealed that H23 in KS89WGRC03 and an unnamed H gene (HWGRC4) in KS89WGRC04, as well as the wheat curl mite resistance gene Cmc4 and a defense response gene Ppo for polyphenol oxidase may be located near H13. These genes comprise a cluster of arthropod resistance or defense response genes. Marker analysis also revealed that a very small intercalary chromosomal segment carrying H13 was transferred from the H13 donor parent to the wheat line Molly.