Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/6/2005
Publication Date: 4/6/2005
Citation: Price, N.P. 2005. Stable isotopic labeling and mass spectrometry: Measurement of metabolic flux in carbohydrate biosynthetic pathways [abstract]. 6th Carbohydrate Bioengineering Meeting, April 3-6, 2005, Barcelona, Spain. Paper L15.
Technical Abstract: The analysis of carbon isotopomers by mass spectrometry and 13C-NMR is a powerful tool for assessing metabolic turnover. Combining this approach with glycomics enables metabolic flux analysis on a global scale (fluxomics), and provides a large number of criteria with which to assess biological perturbations. The aim of this technique is to quantify the fractional incorporation of **13C from exogenous **13C-labeled precursors into all cellular carbohydrates. Tracing carbohydrate metabolic pathways by LC-electrospray-MS, GC-EI-MS, and HSQC-NMR, and new MS techniques using diethyldithioacetal acetates to estimate **13C-flux into the characteristic anomeric carbon-1 of aldoses will be described. The potential of chirally-deuterated (S)-[6'-**2H1]glucose as a tracer will also be discussed. Our current interest includes glycomic flux in human salivary glycoproteins, the bifido pathway in lactic acid bacteria, and biosynthesis of the 11-carbon amino-dialdose, tunicamine.