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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Reproduction Research » Research » Publications at this Location » Publication #170490


item Young, J
item Ashwell, M
item Rohrer, Gary
item Smith, A
item Cassady, J

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2004
Publication Date: 1/15/2005
Citation: Young, J.M., Ashwell, M.S., Rohrer, G.A., Smith, A.L., Cassady, J.P. 2005. Comparative mapping between the pig (sus scrofa) and human. Proc., Plant & Animal Genome XIII, San Diego, CA. P557. p. 210.

Interpretive Summary: Meeting abstract. No interpretive summary required.

Technical Abstract: Sus scrofa chromosome 16 (SSC16) has been associated with reproduction in pigs (Blowe et al. 2004). The follistatin gene has been mapped to SSC16 and is a candidate gene for litter size. The pig map has relatively few markers on SSC16, and there are very few Type 1 markers on SSC16. The objective of this research was to produce a comparative map between SSC16 and Homo sapiens autosome 5 (HSA5). This effort targeted genes which would be predicted to map to SSC16 and which have been previously associated with reproduction in humans. Three genes were selected from HSA5 to use in the comparative mapping process. A fourth gene which maps to SSC16 via radiation hybrid mapping and HSA19 has also been chosen. Human sequence was used to identify a pig EST corresponding to each gene of interest. Primers were then designed to span at least one intron. The resulting amplicon was sequenced, BLASTed (to verify that the correct gene was amplified), and SNPs were identified. The USDA mapping family was then genotyped for the SNPs. We have determined that pig SKP2 maps to SSC16 at an approximate position of 20-25 cM. SNP detection and linkage mapping are underway for the remaining genes.