Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 10/11/2004
Publication Date: 10/11/2004
Citation: Scheffler, B.E., Taliercio, E.W., Scheffler, J.A., Yu, J. 2004. Molecular markers run on a test panel: a data source for the cotton community. International Cotton Genome Initiative Workshop.
Technical Abstract: Unlike other major crops such as soybean and corn, cotton has no coordinated public marker database. Cotton Incorporated (CI) has identified the need for more markers and a database as critical action points. The Mid South Area Genomics Laboratory in cooperation with scientists from the Crop Genetics & Production Research Unit at Stoneville, Mississippi responded and initiated a project to screen and evaluate molecular markers. To facilitate comparison of marker data among different groups, a standardized set of DNA from a range of cotton varieties and wild species was selected to use for the screening. The DNA set is being maintained centrally by the ARS Unit at College Station, Texas, so that future screening projects by other groups can all use the same testing set. Simple Sequence Repeat (SSR) markers, from a variety sources, were screened on the standardized CI DNA panel. Protocols were developed to automate the procedures and minimize the amount of primer, DNA and PCR reaction components required. The Mid South Area Genomics Laboratory is currently working with Clemson University Genomics Institute (cugi) developing a database to make the markers and evaluation data publicly available. The database would allow scientists to search for markers that may be useful for their individual experiments.