Submitted to: International Conference on Microbial Genomes
Publication Type: Abstract Only
Publication Acceptance Date: 9/8/2004
Publication Date: 9/26/2004
Citation: Posada-Buitrago, M., Boore, J.L., Frederick, R.D. 2004. Comparative analysis of expressed sequence tags in the soybean rust pathogen phakopsora pachyrhizi. International Conference on Microbial Genomes. Abstract booklet presented at meeting.
Technical Abstract: The obligate fungal pathogen Phakopsora pachyrhizi causes soybean rust, the most devastating foliar disease in many soybean production areas around the world. The disease has not been reported in the continental United States of America, but recent findings in South America demonstrate that P. pachyrhizi is spreading to new geographic regions, becoming a major threat to U.S. soybean production. The infection process was examined by comparing approximately 16,000 expressed sequences tags (ESTs) generated from three stage-specific unidirectional cDNA libraries from P. pachyrhizi. The EST cDNA libraries were constructed using mRNA isolated from urediniospores germinating on a water surface for 16 h and from infected soybean leaf tissue at 6-8 and 13-15 days post inoculation. Intercellular hyphal growth and the peak of sporulation occur at 6-8 and 13-15 days, respectively. The ESTs were analyzed for redundancy within and among the three libraries, and they were classified according to putative function based on similarity to known proteins using the Blast sequence algorithms. These ESTs will provide insight into the biochemical and physiological processes that are necessary for infection, survival and reproduction.