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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #169941
Title: PEANUT EXPRESSED SEQUENCE TAG (EST) PROJECT AND THE MARKER DEVELOPMENT FOR CULTIVATED PEANUT

Author
item Guo, Baozhu
item LUO, MENG - UNIVERSITY OF GEORGIA
item Dang, Phat
item HE, G - TUSKEGEE UNIVERSITY
item Holbrook, Carl - Corley

Submitted to: International Crop Science Congress Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 4/16/2004
Publication Date: 10/1/2004
Citation: Guo, B., Luo, M., Dang, P. M., He, G., Holbrook, Jr., C. C. 2004. Peanut expressed sequence tag (EST) project and the marker development for cultivated peanut. New Directions for a Diverse Planet: Proceedings for the 4th International Crop Science Congress, September 26 - October 1, 2004, Brisbane, Australia. Available: http://www.cropscience.org.au.

Interpretive Summary: Given its economic and nutritional importance of U.S. peanut, peanut is virtually unexplored at the genomic level because of the peanut genome size (2800 Mb or about the size of the human genome) and the complication. The slow progress in overcoming these factors has impeded the development of intrinsic low-cost and environmentally benign solutions to many challenges that increase the cost and risk of peanut production. An EST (expressed sequence tag) project of cultivated peanut, a part of the U.S. Peanut Genome Initiative supported by U.S. Industry and Peanut Growers, has been successfully initiated at the Crop Protection and Management Research Unit, Tifton, GA, to develop genomic resource for peanut research. We have released the first batch of 1350 ESTs from two cDNA libraries and 100 (GGC)n simple sequence repeats from sequencing 5000 clones of two SSR enriched genomic libraries to GenBank. The application of genomic tools and information in breeding programs would greatly facilitate the genetic enhancement of cultivated peanut.

Technical Abstract: An EST (expressed sequence tag) project of peanut, a part of the U.S. Peanut Genome Initiative supported by U.S. Industry and Peanut Growers, has been successfully initiated at Crop Protection and Management Research Unit, USDA-ARS, Tifton, GA, to develop genomic resource for peanut research. Given its economic and nutritional importance of peanut, peanut is virtually unexplored at the genomic level because of the peanut genome size (2800 Mb or about the size of the human genome) and the complication. There is wide recognition of the need for peanut EST resource. Our EST project has shown the power of developing genomic resource for peanut research community. Expressed sequence tag (EST) libraries for cultivated peanut (Arachis hypogaea L.) were developed from two cDNA libraries constructed using mRNA prepared from leaves of peanut line C34-24 (resistant to leaf spots and tomato spotted wilt virus) and immature pods of peanut line A13 (tolerant to drought stress and preharvest aflatoxin contamination). Randomly selected cDNA clones were partially sequenced to generate a total of 1825 ESTs, 769 from the C34-24 cDNA library and 1056 from the A13 cDNA library, in which 536 and 769 unique ESTs were identified, respectively. We have released the first batch of 1350 ESTs (Genbank accession number CD037499 to CD038843) to GenBank, and recently 100 (GGC)n simple sequence repeats in peanut (accession number AY526357 to AY526456) have been released to GenBank from sequencing 5000 clones of two SSR enriched genomic libraries. Four hundred unigenes have been selected from these ESTs and arrayed on glass slides for gene expression analysis. The application of genomic tools and information in breeding programs would greatly facilitate the genetic enhancement of cultivated peanut.