Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/6/2004
Publication Date: 12/1/2004
Citation: Zhang, X., Zhou, M., Ren, L., Bai, G., Ma, H., Scholten, O.E., Guo, P., Lu, W. 2004. Molecular characterization of fusarium head blight resistance from wheat variety wangshubai. Euphytica. 139:59-64. Interpretive Summary: Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from a Chinese resistant wheat cultivar, Sumai 3, and its derivatives have been well characterized through molecular markers. In this study, DNA molecular markers were used to analyze resistance genes in an old Chinese resistant cultivar, Wangshuibai. We tentatively identified 15 molecular markers associated with three genes for FHB resistance on two chromosomes. One was mapped on chromosome 1B and two others were mapped on 3B. One gene on 3B showed a major effect and explained up to 23.8% of the variation for FHB resistance. Some of the markers can be used for marker-assisted breeding to improve wheat resistance to FHB.
Technical Abstract: Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives, such as Ning 7840, have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F7-derived recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra's'. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs (EcoRI/MseI) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance (p<0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance.