Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #169659


item Waters, Wade
item Palmer, Mitchell
item Bannantine, John
item Whipple, Diana
item Greenwald, Rena
item Esfandiari, Javan
item Pollack, John
item Mcnair, James
item Andersen, Peter
item Lyashchencko, Konstantin

Submitted to: Wildlife Disease Association Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/27/2004
Publication Date: 8/27/2004
Citation: Waters, W.R., Palmer, M.V., Bannantine, J.P., Whipple, D.L., Greenwald, R., Esfandiari, J., Pollack, J.M., Mcnair, J., Andersen, P., Lyashchencko, K. 2004. Antigen recognition by serum antibodies in white-tailed deer (odocoileus virginianus) experimentally infected with mycobacterium bovis[abstract]. Wildlife Disease Association. p. 299.

Interpretive Summary:

Technical Abstract: White-tailed deer (Odocoileus virginianus) have emerged as reservoirs of bovine tuberculosis (TB) in Northern America. For TB surveillance of captive deer, antibody-based assays are particularly attractive because deer are handled only once and immediate processing of the sample is not required. Sera collected sequentially from 24 Mycobacterium bovis-infected and 7 non-infected deer were evaluated by ELISA, immunoblotting, and Multi-Antigen Print Immunoassay (MAPIA) for immunoglobulin specific to M. bovis antigens. Various routes of experimental M. bovis infection, such as intratonsilar inoculation (n = 11), by aerosol (n = 6), and exposure to infected deer (in contact, n = 7), were studied. Upon infection, specific bands of reactivity at ~24-26 kDa, ~33 kDa, ~42 kDa and ~75 kDa to M. bovis whole cell sonicate were detected by immunoblot. Lipoarabinomannan-specific immunoglobulin was detected as early as 36 days postchallenge and responses were detected for 18/19 intratonsilar and in contact infected deer. In MAPIA, sera were tested with 12 native and recombinant antigens coated on nitrocellulose. All 'in contact' infected (8/8) and 10/11 intratonsilarly-infected deer produced antibody reactive with one or more of the recombinant/native antigens. Responses were boosted by injection of tuberculin for intradermal tuberculin skin testing. Additionally, 3/6 deer receiving a very low dose of M. bovis via aerosol exposure produced antibody specific to one or more recombinant proteins. M. bovis was not isolated from 2/3 non-responding aerosol-challenged deer. Of the 12 antigens tested, the most immunodominant protein was MPB83; however, a highly sensitive serodiagnostic test will likely require use of multiple antigens.