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United States Department of Agriculture

Agricultural Research Service

Title: A NOVEL BIOLOGICAL PROCESS TO CONVERT RENEWABLE BIOMASS TO ACETONE AND BUTANOL (AB))

Author
item Qureshi, Nasib
item Ezeji, Thaddeus
item Blaschek, Hans
item Cotta, Michael

Submitted to: American Institute of Chemical Engineers Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 11/12/2004
Publication Date: 11/12/2004
Citation: Qureshi, N., Ezeji, T.C., Blaschek, H.P., Cotta, M.A. 2004. A novel biological process to convert renewable biomass to acetone and butanol (AB) [abstract]. American Institute of Chemical Engineers Annual Meeting. Paper No. 29d.

Interpretive Summary:

Technical Abstract: Butanol is an industrially important fuel and chemical that can be produced from renewable agricultural crops and residues by fermentation. Unfortunately, this fermentation suffers from butanol toxicity, resulting in accumulation of less than 20 gL**-1 butanol in batch reactors. This limits the use of dilute sugar solutions, usually to less than 60 gL**-1, and results in uneconomic recovery of butanol by distillation. Furthermore, butanol recovery by distillation is complicated by its higher boiling point (118 deg C) than water. In order to solve butanol toxicity (to the culture) and recovery problems and make butanol fermentation a commercially viable process, gas stripping, a novel technique to separate butanol was applied to this fermentation. This technique has various advantages over other techniques including adsorption, liquid-liquid extraction, perstraction (membrane assisted extraction), pervaporation, and reverse osmosis as gas stripping does not require membrane or any chemical for butanol recovery. For this application, fermentation gases (CO2 and H2: produced in this fermentation) were used to remove butanol from the reactor simultaneously as acetone and butanol (AB) (acetone is a byproduct of this fermentation) were produced. The objective was to keep butanol below toxic level in the bioreactor so that the culture could utilize more sugar (>45 gL**-1; more than in a typical batch process). In the gas stripping assisted batch reactor, a sugar concentration of 60 gL**-1 was used as opposed to 45 gL**-1 in the nonintegrated batch reactor; thus utilizing 25% more sugar and producing 33.3% more AB. In this process, AB production was more due in part to efficient utilization of acids (reaction intermediates of AB). The reactor productivity was also improved by 110% due to reduced toxicity to the culture and to higher cell concentration. Since gas stripping relieved butanol toxicity and the culture utilized a higher amount of sugar, another fermentation was run where the initial sugar concentration (in the reactor) was increased to over 160 gL**-1. This sugar level is 356% of that used in a typical batch reactor. The fermentation was initiated in a batch mode to produce AB. Gas stripping was started after approximately 40 h of fermentation. As butanol was produced in the system, it (butanol) was recovered simultaneously. As a result of recovery, all the sugar present in the reactor was used thus producing 429% more AB than in a nonintegrated batch reactor. Since it was a closed system, acids were converted to AB, hence improving AB yield by 17.5%. An initial sugar level of higher than 160-170 gL**-1 could not be used due to sugar toxicity to the culture. As a sugar concentration higher than 160-170 gL**-1 could not be used due to sugar inhibition, a fed-batch reactor was initiated with 100 gL**-1 initial glucose and product recovery was initiated after 40 h of fermentation. As the sugar level in the reactor decreased to 20 gL**-1, feeding a concentrated glucose solution (500 gL**-1) was started to keep glucose level in the reactor below inhibitory level (usually 80-90 gL**-1). In this system, 500 g sugar per L culture volume was used, which is over 1100% of that used in the nonintegrated batch reactor. The culture produced 233 g AB as opposed to 17.6 g (per L culture volume) in the nonintegrated system. Examination of results revealed that fermentation possibly stopped after 201 h due to accumulation of nonvolatile components and/or decreased water activity. AB production was 1316% of that in a nonintegrated process. In the end of fermentation, it was observed that the broth became viscous, and the culture started producing acids. To overcome the problem of accumulation of nonvolatile inhibitory components, a continuous system was initiated where a continuous small bleed was withdrawn from the

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