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United States Department of Agriculture

Agricultural Research Service

Title: DEVELOPMENT AND TESTING OF A HIGH-DENSITY CDNA MICROARRAY RESOURCE FOR CATTLE)

Author
item Suchyta, Steven
item Sipkovsky, Sue
item Kruska, Rachael
item Jeffers, Abra
item Mcnulty, Amanda
item Coussens, Matthew
item Tempelman, Robert
item Halgren, Robert
item Saama, Peter
item Bauman, Dale
item Boisclair, Yves
item Burton, Jeanne
item Collier, Robert
item Depeters, Edward
item Ferris, Theodore
item Lucy, Matthew
item Mcguire, Mark
item Medrano, Juan
item Overton, Thomas
item Smith, Timothy - Tim
item Smith, George
item Sonstegard, Tad
item Spain, James
item Spiers, Donald
item Jianbo, Yao
item Coussens, Paul

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 1/10/2004
Publication Date: 1/10/2004
Citation: Suchyta, S.P., Sipkovsky, S., Kruska, R., Jeffers, A., Mcnulty, A., Coussens, M.J., Tempelman, R.J., Halgren, R.A., Saama, P.M., Bauman, D.E., Boisclair, Y.R., Burton, J.L., Collier, R.J., Depeters, E.J., Ferris, T.A., Lucy, M.C., Mcguire, M.A., Medrano, J.F., Overton, T.4., Smith, T.P., Smith, G.W., Sonstegard, T.S., Spain, J.N., Spiers, D.E., Jianbo, Y., Coussens, P.M. 2004. Development and testing of a high-density cDNA microarray resource for cattle. Proc., Plant and Animal Genome Abstracts. P831. p. 278.

Interpretive Summary:

Technical Abstract: A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortiums's (NBFGC) EST collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues. The NBFGC microarry has been tested for false positive rates using self-hybridizations and was shown to yield robust results in test microarray experiments. A web-accessible database has been established to provide pertinent data related to NBFGC clones, including sequence data, BLAST results, and ontology information. The NBFGC microarray represents the largest cDNA microarray for a livestock species prepared to date and should prove to be a valuable tool in studying genome-wide gene expression in cattle.

Last Modified: 8/24/2016
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