Submitted to: Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2004
Publication Date: 8/30/2004
Citation: Johnson-Brousseau, S., McCormick, S.. 2004. A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes. Plant Journal, 39(5):761-775. Interpretive Summary: This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.
Technical Abstract: Here we provide detailed protocols for preparing flowers for microscopic observation of pollen and for collecting pollen in bulk for germination or for RNA or protein isolation. We outline crossing schemes that will help confirm or refute hypothesized roles for the gene of interest, and we outline phenotypic characterizations that are useful in determining whether a gene product is important during one or more phases of pollen development or germination.