Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/14/2003
Publication Date: 5/21/2003
Citation: Sugimoto, M., Ihara, N., Bennett, G.L., Sugimoto, Y. 2003. Eleven previously unreported dinucleotide microsatellite loci on bovine chromosome 19. Animal Genetics. 34:236-237. Interpretive Summary: The location of cattle genetic markers close to cattle genes that are very similar to human genes is important information for identifying the location of additional genes in cattle. Eleven such markers were developed for genes found on cattle chromosome 19 and human chromosome 17. This work adds to the accumulating number of genetic markers that have been located on cattle chromosomes and correspond to genes identified in other species. This information will be important for researchers trying to identify the specific genetic basis for economic differences in cattle.
Technical Abstract: Bacterial artificial chromosome (BAC) clones from the RPCI-42 bovine BAC library positive for known microsatellite loci on bovine chromosome 19 (BTA19) as well as known genes located on human chromosome 17 (HSA17) having conserved synteny with BTA19 were identified by polymerase chain reaction (PCR) amplification. A plasmid library was constructed. The library was screened with 32P-labeled poly-(CA) probes. Positive clones were sequenced and all sequences were queried against GenBank by BLAST searching. Polymerase chain reaction primers were designed interactively with Primer v.3. Polymorphisms at each locus were identified by examining 28 parents of the USDA-MARC cattle reference families. Deoxyribonucleic acid fragments for each locus were amplified and labeled with three fluorescent dyes in the PCR reaction. Polymerase chain reaction products were examined and allele sizes were determined. Four new microsatellite loci (NLBCMK21, 35, 28 and 29) derived from BAC clones containing DIK0423 at 89.8 cM on BTA19 were assigned from 82.3 to 92.0 cM and four other new microsatellite loci (NLBCMK30, 25, 24 and 31) derived from BAC clones containing IDVGA444 at 93.3 cM on BTA19 were assigned from 92.0 to 93.9 cM. NLBCMK39 derived from a BAC clone containing MLL septin-like fusion protein (MSF) assigned to HSA17q25.3 was mapped at 89.8 cM. NLBCMK40 derived from a BAC clone containing SEC14-like 1 (Saccharomyces cerevisiae) (SEC14L1) assigned to HSA17q25.3 was mapped at 91.0 cM. NLBCMK36 derived from a BAC clone containing arylalkylamine N-acetyltransferase (AANAT) assigned to HSA17q25.1 was mapped at 95.0 cM.