|La Rota, M|
|Lapitan, N L|
Submitted to: Wheat Genetics International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2003
Publication Date: 9/1/2003
Citation: Gill, B.S., Qi, L., Echalier, B., Chao, S., Lazo, G.R., Anderson, O.D., Akhunov, E.D., Dvorak, J., Linkiewicz, A.M., Dubcovsky, J., Bermidez-Kandianis, C.E., Greene, R.A., La Rota, M., Sorrells, M.E., Dilbirligi, M., Sidhu, D., Eryman, M., Gill, K.S., Miftahudin, Ma, X., Mahmoud, A., Gustafson, J.P., Wennerlind, E.J., Nduati, V., Gonzalez-Hernandez, L., Anderson, J.A., Peng, J., Lapitan, N.V., Hossain, K., Kalavacharla, V., Kianian, S.F., Pathan, M.S., Nguyen, H.T., Choi, D., Close, T.J., Mcguire, P.E., Qualset, C.O. 2003. A transcriptome map of wheat. In: Proceedings of the Wheat Genetics International Symposium, September 1-6, 2003, Paestum, Italy. p. 261-264. Interpretive Summary:
Technical Abstract: A transcriptome map of wheat genome, with 16,099 expressed sequence tag (EST) loci spanning the 21 chromosomes of bread wheat (Triticum aestivum L.), or an average of 766 loci per chromosome, has been developed. Of the 16,099 EST loci, 14,051 were allocated to 159 bins using a set of nullisomic-tetrasomic, ditelosomic, and deletion lines providing an expected average of 95 loci per chromosome bin, or 1 EST locus per 1Mb of wheat DNA. Observed EST distribution was non-random with 40% of the EST loci mapping in the bins located in the distal 20% of the chromosomes. Most agronomic genes were located in the EST-dense distal bins. The wheat transcriptome map, together with the draft sequence of the rice genome, now provides unique opportunities for gene discovery in wheat. More importantly, the wheat bin map will be a resource for markers for breeding, and a framework for constructing a sequence-ready BAC-contig map of the wheat genome.