Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2002
Publication Date: 1/11/2003
Citation: Anderson, O.D., Anderson, J.A., Close, T.J., Dubcovsky, J., Dvorak, J., Gill, B.S., Gill, K.S., Gustafson, J.P., Kianian, S.F., Lapitan, N., Nguyen, H., Sorrells, M., Steber, C.M., Mcguire, P.E., Qualset, C.O. 2003. Bin-mapping in wheat:toward a physical map of wheat using ests and deletion stocks. XI Plant and Animal Genome Conference Proceedings, January 11-15, 2003, San Diego, California. p. 84. Interpretive Summary:
Technical Abstract: Because of the large size of the wheat genomes, it is unlikely that their actual base-pair sequences will be learned completely in the near future. This four-year NSF PGRP-funded Project, now in its final year, takes an alternative strategy to realize the benefits of new techniques for discovering genes and learning their function. Some 90,000 wheat expressed sequence tags (ESTs) have been generated from 43 cDNA libraries, most of which were created by the Project from diverse wheat tissues produced under numerous controlled conditions. Of these, 10,000 unique ESTs are being identified and mapped to a physical location (bins, defined by deletion stock breakpoints). The sequence and bin location of these ESTs in the wheat chromosomes is publicly available, distributed by means of the website created for this Project (http://wheat.pw.usda.gov/NSF/). The results from this Project are immediately applicable to other crops, because of the close relationship of wheat to other species in the Triticeae tribe and other grass species, especially corn and rice. The diversity of experimental techniques and traits pursued in the individual laboratories collaborating on this Project has made it an ideal training ground for graduate students and post-doctoral scientists. The large pool of well-characterized and mapped unique DNA sequences available in the public domain, is an important resource for future Triticeae research and basic functional genomics research. See the Project website for current status and access.