Author
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Lingle, Sarah |
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Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only Publication Acceptance Date: 8/4/2004 Publication Date: 10/31/2004 Citation: Lingle, S.E. 2004. Expression of Sucrose Metabolism Genes in Developing Internodes of Contrasting Sugarcane Genotypes [abstract]. American Society of Agronomy Annual Meetings [CD-ROM]. Abstract No. 3261. Interpretive Summary: Technical Abstract: There is much variation in how much sucrose different genotypes of sugarcane (Saccharum spp. hybrids) accumulate, but the cause of this variation is poorly understood. Expression of sucrose metabolism genes relative to 18s rRNA was compared in developing internodes of a high sucrose genotype, Muntok Java (MJ), and a low sucrose genotype, PIN84-1 (PIN), using quantitative PCR. In general, expression of all of the genes was greater in MJ than in PIN. This was true for genes of the sucrose cleavage enzymes sucrose synthase (Sus1 and Sus2), soluble acid invertase (Ivr1 and Ivr2), and cell wall acid invertase (Cwin1), as well as genes for sucrose-phosphate synthase (SPS1 and SPS2), which synthesizes sucrose. The sucrose transporter gene, Sut1, had relatively low expression in the very young internode of both genotypes, and expression increased with internode age, especially in the higher sucrose genotype, MJ. The internodes of the sucrose-storing genotype appear to be metabolically more active than those of the low-sucrose genotype. |
