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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #167947
Title: MANAGEMENT OF IN VITRO CULTURES UNDER SLOW GROWTH STORAGE AND IN CRYOBANKS

Author
item ENGELMANN, FLORENT - POLE BIODIVERSITE
item Reed, Barbara
item ENGELS, JAN - IPGR

Submitted to: International Plant Genetic Resources Institute
Publication Type: Book / Chapter
Publication Acceptance Date: 1/1/2003
Publication Date: 1/1/2003
Citation: Engelmann, F., Reed, B.M., Engels, J. 2003. Management of in vitro cultures under slow growth storage and in cryobanks. In: Chaudhury, R., Pandey, R., Malik, S.K., Mal, M. In Vitro Conservation and Cryopreservation of Tropical Fruit Species. p. 229-236.

Interpretive Summary: Collections of valuable plants are often maintained as field plantings. However, slow growth of plant tissue cultures and cryopreservation in liquid nitrogen are increasingly used for storage of germplasm. Efficient, simple and cost effective management procedures are required for these types of germplasm collections. This paper addresses the issues related to management of germplasm collections stored under slow growth and cryopreservation. These guidelines should assist genebank curators with decision criteria and options in establishing, maintaining and using these types of germplasm collections.

Technical Abstract: Slow growth of plant tissue cultures and cryopreservation are increasingly used for storage of germplasm. Slow growth lowers the risk of losing germplasm through handling errors, decreases mislabelling and cuts down on the overall cost of maintaining the germplasm. Efficient, simple and cost effective management procedures are therefore required for these types of germplasm collections. This paper addresses the issues related to management of germplasm collections stored under slow growth and cryopreservation. Only management issues are considered. Adequate management procedures are essential to receive the full benefit of utilization of slow growth storage and cryopreservation for the conservation of plant genetic resources. Guidelines of the management of field and in vitro germplasm collections are currently being vinalized to assist genebank curators with decision criteria and options in establishing, maintaining and using these types of germplasm collections.