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United States Department of Agriculture

Agricultural Research Service

Title: THE USE OF INFECTIOUS CLONES TO EXAMINE CONTRIBUTIONS OF VARIOUS GENES TO VIRULENCE OF NEWCASTLE DISEASE VIRUS)

Author
item Wakamatsu, Nobuko
item King, Daniel
item Seal, Bruce
item Peeters, Ben
item Samal, Siba
item Brown, Corrie

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract only
Publication Acceptance Date: 3/9/2004
Publication Date: 7/24/2004
Citation: Wakamatsu, N., King, D.J., Seal, B.S., Peeters, B., Samal, S., Brown, C. 2004. The use of infectious clones to examine contributions of various genes to virulence of newcastle disease virus. American Association of Avian Pathologists.

Interpretive Summary:

Technical Abstract: Infectious clones of Newcastle disease virus (NDV) were utilized to evaluate relative contributions of the fusion (F) protein, hemagglutinin-neuraminidase (HN) protein, and phosphoprotein (P) to virulence. Specifically, two different clones of the La Sota virus backbone with a F cleavage site mutated to virulent motif, HN chimeras of avirulent La Sota/virulent Beaudette C, and a virulent Beaudette C backbone with mutations in P were examined. Four-week-old White Leghorn chickens were inoculated with viruses, and tissues collected at intervals were examined by histology, immunohistochemistry, and/or in situ hybridization. Birds inoculated with recombinant La Sota with virulent F or virulent (Beaudette C origin) HN genes had histologic lesions and viral distribution that were greater than the lentogenic parent yet

Last Modified: 8/24/2016
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