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United States Department of Agriculture

Agricultural Research Service

Title: INSIGHTS INTO TOLERANCE OF SAG12:IPT PLANTS TO FLOODING USING TRANSCRIPTOME ANALYSIS)

Author
item Vantoai, Tara
item Liu, Fenglong
item Moy, Linda
item Huynh, Le nguyen
item Bock, Geoffrey
item Banowetz, Gary
item Quackenbush, John

Submitted to: Cellular and Molecular Biology of Soybean Biennial Conference
Publication Type: Abstract only
Publication Acceptance Date: 4/20/2004
Publication Date: 8/11/2004
Citation: Vantoai, T.T., Liu, F., Moy, L., Huynh, L., Bock, G., Banowetz, G.M., Quackenbush, J. 2004. Insights into tolerance of sag12:ipt plants to flooding using transcriptome analysis [abstract]. Cellular and Molecular Biology of Soybean Biennial Conference. p. 38.

Interpretive Summary:

Technical Abstract: Transgenic plants (IPT) containing the SAG12:ipt autoregulated cytokinin production capability accumulate more cytokinin and remain greener and produce more biomass and seeds than wild-type (WT) plants under flooding stress. To gain insight into changes in gene expression that contribute to flooding tolerance, whole genome microarray analysis of the 27,000 Arabidopsis genes was conducted at specific times during a 24-hour period of non-flooded control, waterlogging, and complete stress. Hierarchical clustering using Euclidean distance metric revealed distinct patterns of gene expression in these treatments. Under stresses, genes of photosynthesis and energy utilization pathways expressed 2- to 4-fold more in the tolerant IPT plants than in WT plants suggesting that flooding tolerance mechanisms involve both energy production and utilization. Promoter analysis revealed an over-representation of AtMYB2 transcription factor binding motif and putative cis-elements in the differentially expressed genes. Comprehensive analysis of transcriptional changes associated with cytokinin-induced flooding stress tolerance will be reported.

Last Modified: 8/24/2016
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