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Title: GENETIC MAP OF A CROSS BETWEEN FUSARIUM SUBGLUTINANS AND FUSARIUM CIRCINATUM

Author
item BAHLMANN, L - U PRETORIA, S AFRICA
item WINGFIELD, M - U PRETORIA, S AFRICA
item MYBURG, A - U PRETORIA, S AFRICA
item Desjardins, Anne
item GORDON, T - UC DAVIS, CA
item WINGFIELD, B - U PRETORIA, S AFRICA

Submitted to: Mycological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/2004
Publication Date: 7/21/2004
Citation: Bahlmann, L., Wingfield, M.J., Myburg, A.A., Desjardins, A.E., Gordon, T.R., Wingfield, B.D. 2004. Genetic map of a cross between fusarium subglutinans and fusarium circinatum. Mycological Society of America.

Interpretive Summary:

Technical Abstract: The Gibberella fujikuroi complex includes nine different mating populations or biological species (A-I). A genetic map is available for F. verticillioides (mating population A). In a recent study, a cross between F. circinatum and F. subglutinans (mating population H and E) was reported and we have used this cross to map both of these species. This genetic map was constructed using 94 randomly selected ascospore progeny. The mating type (MAT1-1 and MAT1-2) genes, a restriction fragment length polymorphism (RFLP) for the histone (H3) gene and 469 amplified fragment length polymorphisms (AFLP) markers (using 12 primer pairs) were used to generate the map. Using these markers, 12 linkage groups were identified for both the paternal and maternal isolates. The linkage groups ranged in sizes from 30 to 170 cM. Forty-seven percent of all markers displayed transmission ratio distortion. To the best of our knowledge, this is the first study where a hybrid cross between two Fusarium spp. has been used to construct a genetic map of this group of fungi.