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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Insect Behavior and Biocontrol Research » Research » Publications at this Location » Publication #165701

Title: OVIPOSITION AND ISOLATION OF VIABLE EGGS FROM ORIUS INSIDIOSUS IN A PARAFILM AND WATER SUBSTRATE

Author
item Shapiro, Jeffrey
item Ferkovich, Stephen

Submitted to: Florida Entomological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/25/2004
Publication Date: 7/25/2004
Citation: Shapiro, J.P., Ferkovich, S.M. 2004. Oviposition and isolation of viable eggs from orius insidiosus in a parafilm and water substrate. Meeting Abstract.

Interpretive Summary: Not required.

Technical Abstract: To isolate clean intact eggs for use as protein standards in a yolk protein ELISA, an oviposition substrate for O. insidiosus was devised by forming water-filled domes from Parafilm. Eggs deposited in substrates remained viable for at least 24 hr. In no-choice tests 0. insidiosus females oviposited 5.86 eggs/female/day into green beans and 3.06 into water-domes. In a choice test, O. insidiosus females clearly preferred beans to domes (4.02 vs 0.03 eggs/female/18 hr).