Author
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TZANETAKIS, I - OREGONS STATE UNIV |
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Martin, Robert |
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Submitted to: APS Annual Meeting
Publication Type: Abstract Only Publication Acceptance Date: 4/16/2004 Publication Date: 6/11/2004 Citation: Tzanetakis, I.E., Martin, R.R. Complete nucleotide sequence, phylogenetic analysis, and geographic distribution of fragaria chiloensis latent virus, genus ilarvirus. Phytopathology. 2004. v94 p. S104. Interpretive Summary: Technical Abstract: Fragaria chiloensis latent virus (FClLV) is an Ilarvirus first identified in strawberry in 1993 in F. chiloensis plants from Chile. At that time, it was not detected in plants from the Pacific Coast of North America. We have now cloned dsRNA from a plant infected with FClLV. The complete nucleotide sequence of the virus has been determined. RNA 1 encodes for the virus replicase, RNA 2 for the polymerase, and RNA 3 for the movement and coat protein of the virus. Several smaller ORFs are also found in RNA 3, but their expression has not been examined. Phylogenetic analysis reveals that, contrary to previous reports, the virus is closely related to Prune dwarf, Prunus necrotic ringspot, Apple mosaic, and Alfalfa mosaic viruses. Using RT-PCR, the virus has now been detected in strawberry samples from the western United States and Canada, and testing is underway to determine the virus distribution in other areas. It is not clear if the detection with RT-PCR in North America is due to increased sensitivity over ELISA or if the McAb used in the 1993 study was strain specific. |
