Submitted to: Journal of Genetics and Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/18/2003
Publication Date: 11/2/2003
Citation: Hang, A., Burton, C.S., Hoffman, D.L. 2003. Use of intersimple sequence repeat (issr) polymorphisms to study genetic relationships in closely related north american malting barley cultivars.. Journal of Genetics and Breeding.
Interpretive Summary: North American malting barleys consist of two-rowed and six-rowed types. A barley which is accepted for malting must pass several rigorous quality tests established by the malting and brewing industry. Once a malting cultivar or variety is developed, breeders usually use this germplasm again and again in their breeding program in order to maintain the quality. Most of the malting barley therefore is derived from very narrow gene pools and is similar in morphology. Molecular markers can be used to distinguish between different types of cultivars. We used molecular markers generated by twenty intersimple sequence repeat primers to characterize and to study the genetic relationship among 19 two-rowed and six-rowed North American barley cultivars. We were able to differentiate two-rowed from six-rowed types and to plot close relationships among different cultivars. Molecular markers therefore are useful in genetic studies and can be used to select specific characteristics in a breeding program.
Technical Abstract: One hundred intersimple sequence repeat (ISSR) primers were used to study the genetic similarity of nineteen two and six-rowed North American malting barley cultivars. Twenty of the primers yielded a total of 105 bands, thirty-six of which were polymorphic. Cluster analysis of the similarity coefficients among these cultivars revealed that the seven two-rowed cultivars formed a well defined cluster while the twelve six-rowed cultivars formed another distinct group based on their genetic similarity. ISSR analysis is reliable, simple, cost-effective, and can be used effectively both for genome analysis and for marker-assisted breeding selection studies.